Nucleic Acid Extraction Kit Ffpe Genomic DNA Extraction Kit

• CAS No.: 77-86-1 , 7732-18-5, 593-84-0, 1317-61-9
• Formula: /
• EINECS: /
• Classification: Specific Reagents
• Grade: LR
• Specific Usage: For Biological Purpose
• Model NO.: BW-MGD2212-A00
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents
• Source: Self-Prepared Solutions
• Habit Appellation: First Kind of Reagent
• Application: Scientific Research, Research Reagent
• Property: Biochemical Reagent
• Sample: Available
• Color: Transparent
• Appearance: Liquid
• MOQ: 10 Boxes
• Brand: Beiwo
• Storage: Room Temperature (15-25 )12 Months.Long Time
• Product Name: Ffpe Gdna Isolation Kit (Beads)
• Package: Box
• Certificate: ISO MSDS CE
• Transport Package: Carton
• Specification: 50 test
• Trademark: BEIWO
• Origin: Zhejiang, China
• HS Code: 38159000

Product Description

FFPE gDNA Extraction Kit (Beads)
(BW-MGD2212)

 

Product Description

The FFPE gDNA Isolation Kit (BW-MGD2212) is designed for genomic DNA purification from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Instead of using xylene, the kit utilizes a proprietary nontoxic deparaffinization reagent for safe and convenient paraffin removal. To overcome the cross linking of nucleic acids caused by formalin fixation, specially formulated buffers and lysing conditions are developed to release DNA from tissue sections. The purified genomic DNA is suitable for downstream applications such as quantitative real-time RT-PCR, sequencing and mutation screening. This kit is compatible with various high-throughput automated platforms, such as Allsheng Auto-Pure 32A, Allsheng Auto-Pure 96, Thermo KingFisher and so on.

 

KIT CONTENTS

Catalog#	BW-MGD2212-A00-00	BW-MGD2212-A00-01	BW-MGD2212-A00-02
Preps	50	250	1000
FFPE Beads	0.6 mL	3 mL	12 mL
FFPE A	6 mL	30 mL	120 mL
Buffer TL	30 mL	150 mL	3 x 200 mL
Buffer FBL	12 mL	60 mL	240 mL
Buffer MKB	35 mL	170 mL	2 x 350 mL
DNA Wash Buffer*	15 mL	72 mL	3 x 96 mL
Buffer MEB	6 mL	30 mL	120 mL
L solution	120 μL	600 μL	2.4 mL
Proteinase K	1.2 mL	6 mL	24 mL
RNase A (20 mg/mL)	550 μL	2.7 mL	12 mL
User Manual	1	1	1

*Add 60 mL (BW-MGD2212-A00-00) or 288 mL (BW-MGD2212-A00-01) or 384 mL (BW-MGD2212-A00-02) 96-100% ethanol to each DNA Wash Buffer bottle before use.
 

Test Principle During the nucleic acid extraction process, the magnetic bead adsorption principle is used to adsorb, transfer, and purify nucleic acids through special magnetic beads to automatically complete the nucleic acid extraction.

Samples available for extraction
Formalin-fixed, paraffin-embedded (FFPE) tissue samples

Experimental description
Three different kinds of paraffin embedded sections were used as samples and genomic DNA was extracted. Compared with the competitive products, the average molecular weight of the genome extracted by BW-MGD2121 kit was higher.

Features
Instead of using xylene, the kit utilizes a proprietary nontoxic deparaffinization reagent for safe and convenient paraffin removal.This kit is compatible with BEIWO BW Express 16, Allsheng Auto-Pure 32A and other similar nucleic acid purification systems.

Before Starting
Prepare all components and get all necessary materials ready by examining this user manual and become familiar with each step and pay special attention to the followings.

Important Notes
Add 60 mL (BW-MGD2212-A00-00) or 288 mL (BW-MGD2212-A00-01) or 384 mL (BW-MGD2212-A00-02) 96-100% ethanol to each DNA Wash Buffer bottle before use. Add L solution to aliquot of Buffer FBL at 1:100 (V:V) and store at 4. The Buffer FBL/L solution mixture is stable for 1 month at 4.

Materials not Supplied
Sterile collection tubes. Sterile microfuge tubes. Water bath (98/55/65).

Storage and Stability
4-28,avoid direct sunlight. Freezing is prohibited. The kit should be stored dry and at room temperature (15~25°C). They can be stored for at least 12 months without showing any significant reduction in performance, capacity, or quality of separation.

Company Profile

Hangzhou Beiwo Medical Technology Co., Ltd.

      Beiwo was founded in 2018 and headquartered in Hangzhou, China with sister company, Biomiga Inc. located in San Diego, USA. Beiwo is based in the fields of molecular biology and diagnostics, and has a unique "product + service" model. Beiwo's partners include UWI, Genescript,Peking University, Tsinghua University, Zhejiang University, UCSD and other well-known institutions.
      Beiwo has a R&D team consisting of experts from wordwide, and has established a high-standard 100,000-grade GMP production workshop and obtained the ISO9001 quality management system certification.
      Based on the business philosophy of "trust comes from quality". Beiwo Medical focuses on global needs and is dedicated to providing customers with quality products and good technical services.

Our Advantages

Certifications

FAQ

Q: What kinds of products do you have?
A: sample preparation and purification, analysis and detection, BEIWO has corresponding reagents, instruments and even automation platform for scientific research institutions, academic institutions and industrial customers in the biopharmaceutical industry.

Q: How can I get the sample to check your quality?
A: We can ship the sample by international express like FEDEX, DHL etc, or we can choose the appropriate express logistics for you according to your requirements. Sample and freight cost will be needed.

Q: Can you provide different specifications of the kits according to the quantity we need?
A: Yes, we can provide the kits according your requirements, and we also can do ODM and OEM.

Q: What's your MOQ?
A: The MOQ for kits is 10 boxes. If some samples are needed, the total number of samples cannot be less than 5 boxes. For customized products, you can set the scales with your specification, logo, packing, etc, so the MOQ shall be negotiated.

Q: Do you have any certificates?
A: Yes, we have the certificatesISO9001, CErequired for export and local import custom clearance.

Q: What's your production capacity?
A: We have the production capacity of 50 thousand tests of kits per month.

Q: What's your lead time?
A: Our lead time is 5-10 days, if you need a large amount, the lead time may be 7-15 days, if more, then the exact time can be negotiated.

Q: What are your method of payment?
A: We support L/C, D/P, or T/T to business account.

Packaging & Shipping